ABSTRACT
Leaf anatomical traits play an important role in the taxonomic delimitation of species and its relation to adaptation to the environment such as arid or semiarid conditions. In this technique, a small portion of leaf lamina of each species is put in a mixture of 10% chromic acid: 10% nitric acid in a test tube and the mouth of the test tube is plugged with cotton. Petiole plays a vital role in the transport of nutrients and water to the leaves and provides support to the leaf lamina as well as protruding leaves to solar radiation for photosynthesis. Leaf anatomical traits have been used in the taxonomic delimitation. Leaf venation in a plant represents a typical architectural system of vascular bundle traversing through leaf lamina starting from a petiole. It serves two important functions, offering mechanical strength and transport of water, nutrients, and assimilates, besides hormones. Depending on the xylem structure, the sections are in general trans-versal, radial, and tangential.
