ABSTRACT
Surface plasmon resonance (SPR) systems offer a set of advantages for label-free recognition of biomolecular associations. These systems have broad applications in many fields, but find their main use in biosensing, materials screening, and drug discovery. The use of SPR for the analysis of various processes occurring in the range of units, tens, or hundreds of nanometers from the surface is based on the strong dependence of the resonance conditions on the optical parameters of the medium in direct contact with the metal. Besides the optimization of sensitivity, stability, and reproducibility of the physical transducer, one also needs to prevent the denaturation of biomolecules on the surface and to prepare the conditions for the formation of organized structures on the rough surface of polycrystalline films. By applying SPR phenomenon to the development of measuring systems, it is possible to make cheap, compact, and express biosensors high acceptable at the market.
