ABSTRACT
Anise (Pimpinella anisum L.) is one of the most important annual medicinal plants from Apiaceae family in the world. It is a dainty, white-flowered plant, about 44 cm high, native of Iran, Turkey, Egypt, Greece, Crete and Asia Minor. Its active substances are used in various pharmaceutical and food industries (Nabizadeh et al., 2012). It is widely distributed and mainly cultivated for the seeds. The seeds of anise contain an essential oil (1 to 4%) and active substances like anethole used in various pharmaceutical and food industries (Klaus et al., 2009). It is a folkloric remedy for seizures in traditional medicine (Pourgholami et al., 1999; Staub, 2008; Özel, 2009; Aćimović, 2013).
