ABSTRACT
This chapter presents the results of specific practical studies of biomolecular interactions with surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR) techniques. The first study deals with SPR measurement of IgG-anti-IgG immunological reaction and calculation of surface molecular concentration and mass of respective biomolecular coverings. The second study is focused on developing an LSPR sensor based on gold nanoparticle arrays fabricated using nanoimprint lithography and reports sensor capability to monitor antigen-antibody specific reactions between bovine serum albumin (BSA) and anti-BSA immunoglobulin. Biospecific reactions were investigated between the complementary (biospecific) pairs of IgG-anti-IgG immunoglobulins and between protein G and IgG in the flow mode using phosphate-buffered saline as a buffer solution. To evaluate the kinetic response of the LSPR biosensor to the adsorption of real biological materials, nanoparticle arrays were used to detect a biospecific reaction between the protein BSA and anti-BSA immunoglobulin.
