ABSTRACT
The cloning of genes is often a crucial step in a scientific project and can be both difficult and time-consuming. The use of PCR has greatly enhanced the successes of gene isolation. Cloning of genes by PCR can be divided into two main areas: (i) genes of known DNA sequence; and (ii) genes of unknown DNA sequence. Genome sequencing projects (Chapter 10) are generating an increasing amount of data that makes cloning of genes more straightforward; however there remain many cases where unknown genes must be cloned. This chapter deals with the cloning of both unknown genes and those that have been previously isolated.
