ABSTRACT
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Cell Cultures
HEK
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INTRODUCTION
WKH 86 $UP\ 0HGLFDO 5HVHDUFK ,QVWLWXWH RI &KHPLFDO LQFOXGHV EDVLF UHVHDUFK LQWR WKH PHFKDQLVPV RI DFWLRQ RI QWV7KHUHVXOWVREWDLQHGIURPVXFKVWXGLHVDUHWKHQXVHGWR LFDOLQWHUYHQWLRQVWUDWHJLHVWRSURWHFWZDUÀJKWHUVDQGFLYLO E\ WKHVH DJHQWV 7KH FHOOXODU SKDUPDFRORJ\ WHDP DW WKH UHVHDUFKRQWKHDFWLYHFHOOXODUDQGWLVVXHSDWKRJHQLFPHFK ZLQJH[SRVXUH WR VXOIXUPXVWDUG dGLFKORURHWK\O VXOÀGH '7KLVUHSRUWSUHVHQWVVRPHRIWKHUHVXOWVRIWKRVHVWXGLHV VSHUWDLQLQJWRPRGHOVDQGSURFHGXUHVHPSOR\HG
MATERIALS AND METHODS
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Lymphocytes
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DNA Isolation
Reagents
7KHIROORZLQJEX
Phenol-Chlorofor
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Commercial Kits
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m Extraction
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Modified Kit Usag
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Pulse Field Gel E
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PARP Assay
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Calcium Measure
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e
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ment
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Measurement of F
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c and C1q Receptors
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RESULTS
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DISCUSSION
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REFERENCES
