ABSTRACT

A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. A bestseller in its first edition, this second edition provides the tools for developing innovative approaches to using this leading technology. It includes theoretical co

part |71 pages

Section I General Considerations

chapter 2|9 pages

Long PCR from Damaged DNA

chapter 5|9 pages

Pre-PCR Processing Strategies

chapter |1 pages

PCR Optimization

chapter |1 pages

Labeling and Cloning

part |1 pages

Section II Analytical Applications

chapter 10|12 pages

High-Throughput Genotyping and the PCR

chapter 18|8 pages

Pyrosequencing Technology

chapter 19|7 pages

Octamer-Primed Cycle Sequencing

chapter 20|7 pages

In Situ Amplification of cDNA

chapter |1 pages

Nucleic Acid Quantitation

chapter 29|7 pages

Suppression Subtractive Hybridization PCR

part |1 pages

Section III Preparative Application

chapter 31|5 pages

Chromosome Walking by Inverse PCR

chapter 32|6 pages

Long Universal Fast Walking

chapter |1 pages

In Vitro Evolution