ABSTRACT

Apart, from these, the main reason often being the lack of enzyme stability at elevated temperatures and rigorous process conditions. One approach by which this limitation is overcome is by cross-linking the enzymes and crystallizing these cross-linked enzyme systems. These are termed cross-linked enzyme systems, or CLECs. Cross-linking “locks” the protein in a particular conformation. Historically, glutaraldehyde (1) has been by far the most popular cross-linking agent. However, a number of other reagents are now known (Fig. 6.1).