ABSTRACT
Human apoE3 and apoE4 transgenic mice were generated on an apoE-deficient C57BL/6J background utilizing human apoE3 and apoE4 transgenic constructs as previously described.13
The experiments were performed with the apoE3-453 and apoE4-81 lineages which express similar levels of brain apoE and which were back-bred with genetically homogeneous apoEdeficient mice (Jackson Laboratories, Bar Harbor, ME, USA; catalog no. N10JAX) for more than ten generations. The mice, which were heterozygous for the human apoE transgene and homozygous for mouse apoE deficiency, were genotyped by polymerase chain reaction (PCR) as previously described.8
