ABSTRACT
Various methods have been employed for embryo sexing, such as separation on the basis of sexual dimorphism, detection of sex chromatin mass, fluorescence in situ hybridization, detection of the HY antigen and use of a Y-specific DNA probe. Several processes are involved in nuclear transfer including use of a particle gun, sperm-mediated gene transfer, injection of the pronuclei, retroviral-mediated gene transfer, injection of the oocyte cytoplasm and germinal vesicle gene injection.
