ABSTRACT
Chromatography is a highly selective separation technique commonly used for the isolation and purification of biologicals. In chromatography, a liquid mixture is passed through a column of packed particles. Because solutes in the mixture have different molecular size vis a vis the pore volume of the matrix beads or affinities for the sorbent, they partition differently in the stationary sorbent phase and in the mobile liquid phase. Those molecules of a smaller size or a greater affinity for the solid phase (i.e. due to the charge distribution, hydrophobicity, or hydrogen bonds) migrate slower than larger molecules or those attracted to the mobile phase, thus effecting resolution. The column based dynamic processes can, in some cases be replaced by a batch mode of retention based on the same principles.
