ABSTRACT

As with manual assessment, slide preparation and staining techniques for sperm morphology can have a significant influence on the recognition, evaluation, and precision of automated systems. The possible variation that can arise from these steps must therefore be minimized, by selecting methods that optimize automated analysis. The production of reproducible and readable slides to ensure reliable results is, for obvious reasons, more critical for computerized evaluations that for manual evaluations of sperm morphology1,2,3.