ABSTRACT
Introduction ..........................................................................................................365 Protocol 1. Culture of ES Cells in Media Containing Leukemia Inhibitory Factor (LIF) ..........................................................................................................366 Protocol 2. Preparation of Mitotically Inactivated STO Cells as Feeder Layers for Growth of ES Cells ........................................................................................367 Protocol 3. Culture of ES Cells on Fibroblast Feeder Layers .........................368 Protocol 4. Trypsinizing, Freezing and Thawing of Mammalian Cells ...........369
