ABSTRACT
This technique detects specific DNA sequences and is used to analyze gene structure. DNA is digested with a restriction enzyme and the fragments are separated by size on an agarose gel. The gel is blotted by capillary action and the DNA fragments are transferred to a membrane where it is hybridized with a radiolabeled probe. The membrane is washed to remove unbound probe. Exposure of the washed membrane to X-ray film produces bands corresponding to hybridizing DNA fragments whose lengths can be estimated from their position on the membrane.
