ABSTRACT

Concentration methods that have been developed were designed to improve one's ability to find eggs and/or cysts of parasites. Some procedures or modifications of concentration methods fall into two general categories: flotation and sedimentation methods. Brine Flotation, introduced by Bass in 1906, consists of floating eggs and cysts in a saturated salt solution and is still used in veterinary practice. It can be used for concentrating various eggs and coccidia, including Cryptosporidia, from fresh feces. The concentration method using a saturated sugar solution and centrifugation proposed by Sheather in 1923 is considered to be a better method by most workers. Although other methods were better for eggs of a specific group of parasites such as the schistosomes, of all concentration methods tested, formalin-ether sedimentation gave the best overall results for concentrating all of the different kinds of eggs and cysts, especially when different kinds of eggs and cysts were present in the same specimen.