ABSTRACT
This chapter presents some efforts to develop an immunoisolation system for organelle fractionation. It describes a new free-flow immunoisolation system designed to reduce the stress applied to the isolated material. Subcellular fractionation is monitored using the evaluation criteria of yield and relative specific activity calculated from a balance sheet of the fractionation. The shape of the magnetic field has been designed to permit the beads to be maintained in a disperse suspension and to be contained within the chamber while a buffer flows through the chamber. The specific antibody is always the most important reagent of the immunoisolation system. An additional advantage of a linker molecule is to provide flexibility which gives the antibody a better chance to find its antigen. The input fraction varies considerably depending on the experimental system. Aggregation of the beads may occur in the magnetic field.
