ABSTRACT

Color and fluorescent reactions have been considered in the application to study location of neurotransmitters in plant cells as well as binding of their agonists and antagonists. Model systems for the investigations served both intact cells as well as isolated organelles (nuclei, vacuoles, and chloroplasts). Microscopic analysis included usage of (1) azo dyes demonstrating color reactions were recommended to show a location of cholinesterase as marker of acetylcholine and (2) fluorescence reactions for the observation neurotransmitters in situ. The application of fluorescent agonists and antagonists of neurotransmitters showed possibilities to analyze the presence of their receptors.