This chapter discusses the common sources of infection in sleep laboratory. It takes optimal precautions for control of infections in sleep laboratory. The chapter discusses the common modes of infection transmission and their prevention measures. Infection control is an important issue during the sleep study. A patient has to spend two nights in a hospital room. In addition, a number of instruments are applied during the study, and these may transmit infection. The first source of infection could be the room itself. This is an example of airborne infection. The second common source of infection is the linen. All linen used in a case study should be thoroughly washed and dried and each patient should be given fresh linen. The other common sources of infection include the nasal cannula, mask of positive airway pressure (PAP), and hosepipe of the PAP machine.