ABSTRACT
This section should be regarded as a supplement to Section I.V in CRC Handbook of Chromatography: Carbohydrates, Volume I because there have been few advances in the electrophoretic techniques applied to carbohydrates since the compilation of the earlier volume. In paper electrophoresis sodium metavanadate has been added to the list of com-plexing electrolytes capable of resolving low-molecular-weight carbohydrates, especially alditols, and electrophoresis on glass-fiber strips seems likely to be superseded by the use of silanized silica gel plates, for which some preliminary results are presented here. Changes in the techniques adopted in electrophoresis of glycosaminoglycuronans on both cellulose acetate membranes and agarose gels have improved resolution to the extent that heterogeneity of heparin and heparan sulfate has become detectable by the resolution of various components. Discontinuous poly aery lamide gel electrophoresis in the presence of denaturing agents, notably sodium dodecyl sulfate, which has assumed great importance in analysis of glycoproteins, is reviewed at the end of this section; as this technique was not included in Volume I, the review in this case covers literature from 1970 onward.
