ABSTRACT
Supercritical fluid chromatography (SFC) and supercritical fluid extraction (SFE) are two complementary techniques of potential, and in selected settings of practical interest to analysts in toxicology and therapeutic drug monitoring. This chapter examines the roles of SFC by describing the basic principles of SFC, updating the available instrumentations and technologies, and providing the readers with selected applications that may be of clinical and/or research interest to toxicology. Supercritical Fluid provides the medium for the partition processes of the analytes in SFC and SFE. In packed-column application, selectivity of the stationary phase is changed by a low concentration of modifiers, such as methanol, water, formic acid, formamide, acetonitrile, methylene chloride, tetrahydrofuran, and citric acid. Capillary column offers high efficiency and sensitivity, low flow rate, a wide range of detectors, and density programming but accepts limited injection volume.
