ABSTRACT
This chapter describes three kinds of immunomethods—radial immunodiffusion, rocket immune-electrophoresis, and 2D-immunoelectrophoresis—which were considered of interest to study the interactions of proteins with nanomedicines in colloidal dispersion. Prior to colloidal nanosystems, transference to pharmaceutical industries and their commercialization, and after a wide characterization of their physicochemical properties, a deep study of their interaction with biological components is required to ensure safe nanomedicines are obtained and to clearly define their behavior in physiological conditions. Remarkably, not only the therapeutic applications of nanomedicines but their applications as diagnostic nanosystems and even for theranostic purposes combination of therapy and diagnosis in the same nanosystem are of interest. The chapter aims to summarize the main techniques concerning the interaction of nanomedicines with biological systems. It reviews the techniques for a complete characterization of the interaction of nanomaterials with biological components. Colloidal nanosystem cytotoxicity is a key parameter that must be studied when initiating in vitro assays, before any preclinical studies.
