ABSTRACT
High-performance liquid chromatography (HPLC) is a very useful and versatile technique that can be used for the separation and identification of a large variety of organic and inorganic compounds. There has been a tremendous amount of significant developments in the field of HPLC, and at this point in time, some believe that HPLC is rapidly becoming a mature science with all the major problems already solved. There are several HPLC detectors being used, the more popular including ultraviolet ultraviolet -visible, fluorescence, electrochemical, refractive index, plasma emission, and mass spectrometry. Although the synthetic organic literature has many examples of the use of supported reagents for a variety of reasons, the use of supported derivatization reagents or solid-phase reagents used in conjunction with HPLC instrumentation is limited. Obviously, on-line reactions are more desirable than off-line reactions, but off-line solid-supported reactions still have several advantages over off-line solution reactions.
