ABSTRACT
The transposable element in the DNA sequence is capable of moving throughout the genome due to its inbuilt capacity of excision and reintegration. Most of the transposons have inverted repeats at the two ends, with insertion sites as well. The activation of a transposon may be generated by specific elements as in Ac locus in maize mutation or by genomic stress, and its movement facilitated by the enzyme transposase coded in the system. One of the possible approaches towards identification of new genes is by DNA homology—with the conserved nucleotide sequence as the homotype. Another method involves a screening for gene expression patterns using panels of monoclonal antibodies raised against specific parts/metabolite of the organism. Grow transgenic plants by hydroponics, in sand or in agar, in sterile condition, so that the supporting medium can be cleanly removed. Wash away the supporting medium from the roots with double distilled sterile water. Briefly blot the roots and dry.
