ABSTRACT
The cultivation of cells, tissues and organs in vitro forms the theme of tissue culture, where the objective is to simulate in vivo conditions in artificial medium. The technique has immense application in chromosome research, including their manipulation and engineering. In genetics, cell, tissue, and embryo culture has acquired the status of a routine technique in the laboratory. Due to the restricted nature of growth in certain specialized and local regions, not all the tissues and organs form convenient material for cultural studies. The highly active growing points, such as apices of stem, leaf and root as well as buds, provide excellent materials for tissue culture. The term ‘cell cycle’ implies the sequential occurrence of different phases of the cell, initiated from a division till the completion of the next cell division. For chromosome analysis from liquid medium if necessary, the medium should be centrifuged and pellet of cells taken.
