ABSTRACT
The convenience of securing quantitative results is the principal reason for the development of interference microscopy. The light is split into two beams by a beamsplitting mirror, one beam being transmitted through the object and the other passing some distance to the side of it. Ultrastructural analysis of chromosomes is carried out through electron microscope which brings out finer details at a level beyond the resolution of the light microscope. In electron microscopy, electron or magnetic fields are shaped to refract electrons producing the image. The image of electrons is transformed to light image through a fluorescent screen. The image can be viewed on a fluorescent screen or in a photograph. In order to observe with the help of the electron microscope, ultrathin sectioning is necessary, which is cut in an ultramicrotome. The embedding medium also contains a freshly prepared accelerator or activator for infiltration. The final embedding is carried out in polyethylene or gelatin capsules.
