ABSTRACT
Chemical analysis of chromosomes in situ can be carried out through the use of radio-isotopes and estimation of nuclear deoxyribonucleic acid. The objective of autoradiography is to locate radio-active material in a specimen with the help of photography. In autoradiography, the radioactive material is detected by a photographic process of development. The emulsion technique involves the application of the emulsion in the liquid form and has been found to be most suitable for autoradiography. It is widely used for plant, animal and human tissues and also for their cultures and allows the formation of a monolayer of emulsion on the tissue. High resolution technique and its combination with immunofluorescence have allowed the delimitation of functionally specialized segments of chromosomes. The principal difference between an ordinary light microscope and an ultraviolet microscope lies in the fact that in the latter, transparent fused quartz lenses are used in place of optical glasses, which are opaque to shorter ultraviolet wavelengths.
