ABSTRACT

This chapter provides a short introduction to the main detectors used in supercritical fluid chromatography (SFC) with a special emphasis to mass spectrometry by David Pinkston, the Fourier transform infrared spectrometry by Philippe Morin and evaporative light scattering detection by Michel Lafosse as they have special features that deserve for their use in SFC. SFC chromatograph used for hyphenation to atmospheric pressure detection such as flame ionization detector (FID) is very similar to a gas chromatography apparatus. When no splitting of the mobile phase is required, in capillary and micropacked column SFC, SFC-FID is straightforward whatever the structure of the solutes. Using the low pressure detection scheme, fluorescence detection limit of 45 fmol of diphenylanthracene was reported. High performance liquid chromatography coupled to Nuclear Magnetic Resonance is starting to become an established hyphenated technique as suggested by the results presented by many groups in the last symposiums on chromatography.