ABSTRACT
The separation of the troponin complex of muscle was first accomplished by S. Ebashi. Since the bovine cardiac troponin T variants arise from the deletion of charged residues, the method best suited to the separation of such variants would be based on ion-exchange chromatography (IEC). The diagnosis of renal transplant rejection, bilary obstruction, and some forms of cancer, are now possible by monitoring the presence of isoenzymes using ion-exchange high-performance liquid chromatography (HPLC) methodology. Employ open-column IEC which may be of sufficient resolving power to isolate only the most abundant protein isoforms. Electrophoretic screening has in the past been the most common method, along with isoelectric focusing and open-column chromatographic procedures, for the identification of variants. The initial purification of these troponin complexes by ion-exchange HPLC used a combination of both anion-exchange and cation-exchange analytical columns. Separation of the bovine and rabbit complexes exhibited other differences when the results from the weak cation-exchange column were analyzed by gel electrophoresis.
