ABSTRACT
This chapter provides an outline of the general methods of recombinant deoxyribonucleic acid (DNA) technology and its application. Recombinant DNA technology is a procedure by which a fragment of DNA from one organism can be cloned into another, or even into the same organism. Recombinant DNA technology would not have progressed without the discovery and availability of restriction endonucleases. A vector with foreign DNA can be introduced into cells by a variety of procedures. A wide variety of procedures can be used to isolate and to identify genomic and complementary DNA clones. The DNA or Ribonucleic acid (RNA) used for annealing that is labeled with the radioisotope is called the probe. Hybrid-arrested translation is based on the observation that Messenger RNA (mRNA) molecules must be single stranded to enable translation. Hybrid selection is based on the ability of recombinant plasmids to select the specific mRNA of interest from a population of unfractionated mRNA molecules.
