ABSTRACT

A population of recombinant DNA molecules can be made, each recombinant molecule containing one of the mouse DNA fragments in the original mixture. This can then be introduced into a population of bacteria such that each bacterial cell contains, in general, a different type of recombinant DNA molecule. If we can identify the bacterial cell that contains the recombinant DNA bearing the mouse DNA fragment we want, the cell can be multiplied in culture and large amounts of the recombinant DNA isolated. The mouse DNA of interest can then be recovered from this in pure form; it is then said to have been cloned.