ABSTRACT

Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics.  Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. 

Practical examples taken from the literature demonstrate theory throughout.  The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. 

Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level.  Enzymologists and molecular biologists will find the text a useful reference.

chapter 1|8 pages

Nomenclature

chapter 2|10 pages

Introduction to Kinetics

chapter 3|15 pages

Enzyme Assays

chapter 7|19 pages

Pre-Steady-State and Relaxation Kinetics

chapter 8|27 pages

Isotopic Probes of Kinetic Mechanism

chapter 9|71 pages

Isotope Effects as a Probe of Mechanism