Development and Operation of a Cation-Exchange Chromatography Process for Large-Scale Purification of a Recombinant Antibody Fragment

Ion-exchange chromatography is a widely used method of chromatographic purification. In cation-exchange chromatography, a positively charged protein absorbs onto a negatively charged sorbent, then an increasing salt concentration in the mobile phase selectively desorbs (elutes) the protein components as positive ions in the mobile phase buffer displace the proteins from the sorbent [1]. Cation-exchange chromatography is commonly applied to the purification of antibodies and antibody fragments [2, 3].