ABSTRACT
Reversed-phase liquid chromatography (RPLC) is applicable for separations of a wide variety of solutes with different functionalities. The chromatographic stationary phase contributes essential properties that influence the final outcome of a given chemical separation. Different supports and stationary phases are becoming available that are robust and provide uniquely selective retention behavior for certain particular types of solutes. A variety of physical characteristics of the chromatographic stationary phase affect its final chromatographic performance, including the type and physical properties of the support; the type, quantity, and functionality of the chromatographically active overlayer; and the conditions employed to synthesize the stationary phase. Several techniques have been developed both in the synthesis and characterization of these stationary phases to gain insight into which parameters affect separation quality, and by what mechanisms. This information is being used to develop better, more reproducible stationary phases, with more controlled and predictable retention characteristics.
