ABSTRACT
The purification of compounds by preparative liquid chromatography is performed by a wide spectrum of laboratories. Purification and isolation techniques have evolved from lowresolution to high-resolution separations, led by developments in column technologies and packing materials, and followed by adaptations to pumps, detectors, injectors, and fraction collectors [3]. Low-resolution, large-particle columns still have their place for crude or preliminary purifications, but the trend is clearly toward high-resolution, small-particle column separations. As a result, small-scale preparative LC is performed today even on "analyticalscale" HPLC columns and systems.
