ABSTRACT
The immobilized enzyme displayed on the cell surface is also regarded as a kind of a self-immobilized enzyme on the cell surface; this phenomenon is passed on to daughter cells as long as the plasmid or the integrated gene is retained by the cells. This immobilization system could turn or remake S. cerevisiae into a novel and attractive microorganism as a whole-cell biocatalyst by surface expression of various enzymes, especially when target substrates are not able to be taken up by the cells.
