ABSTRACT
The production of foreign proteins using a selected host with the necessary posttranslational modifications is one of the key successes in modern biotechnology. This methodology allows the industrial production of proteins that were otherwise produced in small quantities. However, the separation and purification of these proteins from the fermentation media constitutes a major bottleneck for the widespread commercialization of recombinant proteins. The major production costs (50-90%) for a typical biological product resides in the purification strategy. There is a need for efficient, effective, and economic large-scale bioseparation techniques to achieve high purity and high recovery while maintaining the biological activity of the molecule.
