ABSTRACT

Preparative layer (planar) chromatography (PLC) is a liquid chromatographic technique in which the solvent-solvent composition migrates through the stationary phase either by capillary action or under the influence of forced flow with the aim of separating compounds in amounts of 10-1000 mg (1). The compounds can be isolated for structure elucidation (IR, UV, MS, 1H-NMR, 13C-NMR, CD, etc.), for various analytical purposes or for determination of biological activity (2).