ABSTRACT
Thin-layer chromatography (TLC) as it is applied today was developed in the 1950s. Standardization of sorbents and layer preparation led to wide application of TLC in analytical laboratories (1, 2). The main advantages of this separation method are its simplicity, low cost, and flexibility, and therefore it is now a general laboratory tool similar to titration, crystallization, and so on. Instrumentation for TLC resulted in automated sample application and development and thus good reproducibility. Modern scanning and video instruments can achieve accurate and precise recording of the separated constituents and quantification of chromatograms.
