Generation of transgenic and knockdown mice with lentiviral vectors and RNAi techniques

Transgenic and knockout/knockdown mice have been invaluable for basic biological studies. Lentiviral vectors and RNAi techniques have provided new ways of producing gene-modified animals for biological studies. In this chapter, we go through the basics of generating transgenic and knockdown mice with these new techniques and give complete protocols to perform lentiviral transgenesis via two different methods.