ABSTRACT
Template Because of the extreme amplification achievable, it has been demonstrated that PCR can sometimes amplify as little as one molecule of starting template. Therefore, any source of DNA that provides one or more target molecules can in principle be used as a template for PCR. This includes DNA prepared from blood, sperm or any other tissue, from older forensic specimens, from ancient biological samples or in the laboratory from bacterial colonies or phage plaques as well as purified DNA. Whatever the source of template DNA, PCR can only be applied if some sequence information is known so that primers can be designed.
