ABSTRACT
We have begun to work on the determination of deoxyribonucleic acid (DNA) adducts in biological samples by electrophore labeling gas chromatography (GC). Our overall analytical strategy consists basically of the following steps: (1) isolate the DNA from the biological sample by conventional techniques; (2) hydrolyze the DNA to bases or nucleosides; (3) isolate the DNA adducts from the bulk of normal DNA components; (4) label the adducts with an electrophore; and (5) use GC with electron capture detection (ECD) or detection by negative ion chemical ionization mass spectrometry (NCI-MS) to quantify the adducts with high sensitivity.
