ABSTRACT
The molecular size of dissociable coenzymes is much less than those of enzyme proteins, which makes immobilization of these coenzymes difficult. An affinity chromatographic reactor (ACR) is a bioreactor with immobilized enzymes which effectively utilizes the dynamic affinity between enzymes and coenzyme for the recycling of dissociable coenzymes. In the former, the coenzyme is consumed in the main reactor, recovered at the coenzyme separator, and regenerated to the original active state in the subreactor. A dynamic immobilization of the coenzyme through dynamic affinity between the coenzyme and the enzymes is expected, depending on the concentrations of the coenzyme and the immobilized enzymes and the dissociation constant between the coenzyme and the enzymes. The principles and the possibilities of ACR, which utilizes the dynamic immobilization of coenzymes through dynamic affinity between the coenzyme and the immobilized enzymes. A moving-bed reactor is used and the bed with immobilized enzymes is moved in a reverse direction to that of the coenzyme.
