ABSTRACT
The basic chromatograph will be first described and the essential parts of the system discussed in some detail. This simple chromatograph can be used for the vast majority of liquid chromatography (LC) analyses and, in fact, has been used, very successfully, in both industry and university. The function of the pump is virtually self-explanatory. As the piston moves backwards and forwards the driving fluid causes the diaphragm to oscillate alternately drawing solvent into the diaphragm chamber and then expelling it to the column. The sample valve is one of the more critical parts of the chromatograph as it must place a sample onto the column with great precision. LC detectors will be discussed in detail in the next chapter but some general aspects of the subject will be considered. The fully equipped liquid chromatograph, at prices, will cost in excess of $20,000 depending on the number of alternative detectors and ancillary apparatus that is purchased with it.
