ABSTRACT
The oriental fruit fly, Bactrocera dorsalis, is a serious pest of fruit crops in the Pacific Basin and a constant threat to the US mainland. White puparium is a selectable marker that has been used to construct the first genetic sex sorting system for the oriental fruit fly. The white puparium genetic sexing system is analogous to that developed in the Mediterranean fruit fly using the white pupae strain. Geographic strains were derived from adults and larvae collected from two sites on Oahu, three on Maui, one on Hawaii, and one on Kauai. Ethyl methane sulfonate (EMS) was used to induce mutations in laboratory strains. EMS was administered to adult males less than three hours of age by exposing them to a 0.03 M solution in sucrose. Gold-grey was isolated during screening of laboratory colonies treated with EMS. Linkage group C had five markers, including amethyst, copper, gold-grey, white eye, and white puparium.
