ABSTRACT
Several experimental models have been developed to simulate the microvascular and parenchymal cell alterations associated with acute inflammation. The contention that oxy-radicals and neutrophils are involved in ischemia-reperfusion is further supported by work using intravital microscopy as a method of neutrophil detection. While there is a large body of evidence implicating neutrophils in reperfusion injury, relatively little is known about the factors that initiate and promote the leukocyte-endothelial cell interactions elicited by ischemia-reperfusion. The contention that oxy-radicals and neutrophils are involved in ischemia-reperfusion is further supported by work using intravital microscopy as a method of neutrophil detection. The intravital microscopy method of detecting neutrophil infiltration in the mesentery subjected to ischemia-reperfusion correlates well with myeloperoxidase measurements in mesenteric venules. However, in addition to quantitating neutrophil infiltration, the intravital microscopy technique allows one to define the influence of ischemia-reperfusion on leukocyte adherence to venular endothelium and the emigration of leukocytes from the circulation.
