ABSTRACT
This chapter discusses the process of tangential flow filtration (TFF) and the physicochemical principles governing its performance and describes the filtration media, equipment, and systems currently available for carrying out TFF. Separation of solids from liquids by filtration is among the oldest chemical engineering unit operations. Tangential flow filtration is also challenging conventional microfiltration in such applications as clarification and cold sterilization of biological media, parenteral liquids, liquid foodstuffs, and beverages. The discussion of polarization dealt with the phenomenon solely in terms of the properties and characteristics of the layer of concentrate deposited on the membrane surface during filtration. All microbial-based recombinant proteins are processed and purified through a combination of extraction, solubilization, membrane filtration, and chromatographic separation. A TFF system requires a minimum number of components to operate and control it effectively. Tangential flow filtration devices to remove virus and virus-like particles use size exclusion by ultrafiltration membranes as the predominant mechanism.
