ABSTRACT
This chapter examines the copy number of yeast genomic DNA sequences homologous to Y3002 and Y3015 insert DNA by quantitative hybridization analysis. Three distinct classes of RNA polymerase are responsible for the transcription of DNA into RNA in eukaryotes. RNA polymerase I synthesizes ribosomal RNA; RNA polymerase II is responsible for the transcription of messenger RNA; and RNA polymerase III synthesizes small RNA’s such as transfer RNA and 5S ribosomal RNA. The factors that influence the successful use of antibody probes to isolate and identify clones from recombinant DNA expression libraries merit further comment. The ideal recombinant DNA expression library would have the potential to produce all single polypeptide antigenic determinants encoded in the genome of interest. The isolation of genes encoding yeast RNA polymerase subunits should facilitate a genetic and biochemical definition of the enzyme’s structure and function in eukaryotes.
