ABSTRACT

To meet the demand of expanding world, food production has to be increased. But, due to the increased use of chemical inputs, fertility of soil is decreasing day by day. To overcome the losses, due to chemical fertilizers, there is an urgent need to explore the potential role of free-living nitrogen-fixing heterotrophic organisms in increasing soil fertility. Interactions between plants and associative nitrogen-fixing bacteria, which are measured a detachment of plant growth-promoting rhizobacteria, are the simplest form of nitrogen-fixing symbiosis.

The number of N2-fixing plant-associated bacteria identified is still growing, but we are far from having a complete view of the ecological impact of these associations. Understanding and optimizing N2-fixing plant bacteria associations have promising prospective for sustainable agriculture. Isolation and characterization of effective and competitive strains tolerant to high temperature, drought, nitrate, acidity, and other abiotic stresses is 202of high priority. There is also a need to build up populations by addition of organic materials as well as repeated inoculation of the desired strains.