ABSTRACT
This chapter focuses on the culturing techniques of Dunaliella in media that support both autotrophic and heterotrophic growth. The simplest medium is seawater enriched with nitrate, phosphate, thiamine plus soil extract, and the most complicated is the ATCC-1194 DV medium with 29 enrichments. Dunaliella species seem to tolerate a latitude of salinity conditions, and grow well under a wide range of nutrient concentrations. Procedures for scaling up cultures to large volumes are outlined. A comprehensive discussion is presented on the growth of Dunaliella in batch, continuous, pH stat, cyclostat, turbidostat, chemostat forms, growth under several environmental variables such as pH, temperature, salinity, light and nutrients such as phosphorus, nitrogen, iron and other metals. Further, applications of Dunaliella as live feed in mariculture, for harvesting commercial sources of bioactive compounds, or in wastewater treatment and production of biofuel are pointed.
