ABSTRACT

The soybean (Glycine max) genome sequencing project began as an interagency project with the DOE’s Joint Genome Institute providing the production sequencing throughput with the NSF and USDA funded groups providing genomic resources and soybean expertise to the project (Jackson

1HudsonAlpha Genome Sequencing Center, 601 Genome Way, Huntsville, AL 35806, USA. 2USDA-ARS Corn Insects and Crop Genetics Research Unit, Ames, IA 50011, USA. 3Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA. 4Arizona Genomics Computational Laboratory, BIO5 Institute, 1657 E. Helen Street, The University of Arizona, Tucson, AZ 85721, USA. *Corresponding author: jschmutz@hudsonalpha.org

et al. 2006). The goal of the project is to produce an accurately sequenced, assembled, and annotated representation of the soybean genome that will form the basis for worldwide soybean genomics research. There are several major steps involved in producing this genome sequence including: sequencing, whole-genome shotgun (WGS) assembly, chromosome-scale assembly, and annotation. Here, we describe the 7x sequence coverage WGS assembly, the preliminary annotation of this assembly, and the efforts toward creating an ordered and oriented chromosome-scale representation of the soybean genome sequence.